Innovations in Mass Spectrometry

Mass spectrometry (MS) is an analytical technique that ionizes chemical species and separates the ions based on their mass to charge ratio. The new advancements in current trends of using mass spectrometry hold promises to address the shortcomings of data-dependent analysis and selected reaction monitoring (SRM) employed in shotgun and targeted proteomics, respectively the advancements includes all-ion fragmentation, Fourier transform-all reaction monitoring, SWATH Acquisition, multiplexed MS/MS, pseudo-SRM (pSRM) and parallel reaction monitoring (PRM).. Mass spectrometry in the analytical technique which  is being used in laboratories by researches and scientist from past 25years.

The search of metabolites which are present in biological samples and the comparison between different samples allow the construction of certain biochemical patterns. The mass spectrometry (MS) methodology applied to the analysis of biological samples makes it possible for the identification of many metabolites. The 100 chromatograms were concatenated in a vector. This vector, which can be plotted as a continuous (2D pseudospectrum), greatly simplifies for one to understand the subsequent dimensional multivariate analysis. To validate the method, samples from two human embryos culture medium were analyzed by high-pressure liquid chromatography–mass spectrometry (HPLC–MS). They work on the principle that many microorganisms have their own unique mass spectral signature based on the particular proteins and peptides that are present in the cells. Identification of unknown peaks in gas chromatography (GC/MS)-based discovery metabolomics is challenging, and remains necessary to permit discovery of novel or unexpected metabolites that may allergic diseases  processes and/or further our understanding of how genotypes relate to phenotypes. Here, we introduce two new technologies and an advances in pharmaceutical analytical methods that can facilitate the identification of unknown peaks. First, we report on a GC/Quadrupole-Orbitrap mass spectrometer that provides high mass accuracy, high resolution, and high sensitivity analyte detection.

  • Metabolomics/Lipidomics: new MS technologies
  • NMR Spectroscopy and NMR in biomedicine
  • Approaches in glycoproteins and glycans
  • MS Approaches in Carbohydrates ,microbes and biomolecule analysis
  • Atom probe tomography
  • Protein phosphorylation and non covalent interaction
  • Advances in isolation, enrichment and separation
  • Structural proteomics and genomics
  • Lipidomics, metabolomics and ultratrace analysis
  • Nano scale and microfluidic separations
  • Hybrid Mass Spectrometry

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