New Approaches in Mass Spectrometry

The search of metabolites which are present in biological samples and the comparison between different samples allow the construction of certain biochemical patterns. The mass spectrometry (MS) methodology applied to the analysis of biological samples makes it possible for the identification of many metabolites. The 100 chromatograms were concatenated in a vector. This vector, which can be plotted as a continuous (2D pseudospectrum), greatly simplifies for one to understand the subsequent dimensional multivariate analysis. To validate the method, samples from two human embryos culture medium were analyzed by high-pressure liquid chromatographymass spectrometry (HPLC–MS). They work on the principle that many microorganisms have their own unique mass spectral signature based on the particular proteins and peptides that are present in the cells. Identification of unknown peaks in      gas chromatography (GC/MS)-based discovery metabolomics is challenging, and remains necessary to permit discovery of novel or unexpected metabolites that may allergic diseases processes and/or further our understanding of how genotypes relate to phenotypes. Here, we introduce two new technologies and advances in pharmaceutical analytical methods that can facilitate the identification of unknown peaks. First, we report on a GC/Quadrupole-Orbitrap mass spectrometer that provides high mass accuracy, high resolution, and high sensitivity analytic detection.

  • Metabolomics/Lipidomics: New MS technologies
  • Emerging Separation Technologies
  • Hybrid Mass Spectrometry
  • Approaches in Glycoproteins and Glycans
  • MS Approaches in Carbohydrates, Microbes and Biomolecule Analysis
  • Atom Probe Tomography
  • Protein Phosphorylation and Non-Covalent Interaction
  • Advances in Isolation, Enrichment and Separation
  • Lipidomics, Metabolomics and Ultratrace Analysis
  • Nano Scale and Microfluidic Separations
  • Complementary Techniques and Multitechnique Approaches (XPS, GD-MS, ...)

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