Biography
Biography: Eduard Rogatsky
Abstract
In a past decade in a literature were addressed different methodological questions, related to data variability between immunoassays and LC/MS methods (during clinical assay standardizations studies). However, reasons of inter-lab proficiency testing variability, performed only on LC/MS instruments typically are not discussed. While a pipetations inaccuracy limit is typically within 3% interval, reported proficiency testing results produced by well validated LC/MS methods, could be exceeding 20% bias or ± 3S consensus interval, even if the same method and same instrument brand were used in other laboratories. I present case studies demonstrating impact of calibration curve design, instrument settings, LC and MS instrumental conditions and analyte source chemistry on test results of specimens, blanks and quality controls.