Kashyap N. Thummar
Saurashtra University, India
Title: Analysis of Topiramate in human plasma using liquid chromatography – tandem mass spectrometry and its application to pharmacokinetic study
Biography
Biography: Kashyap N. Thummar
Abstract
A simple, sensitive and reliable liquid chromatography – tandem mass spectrometry (LC –MS/MS) method was developed for the quantiï¬cation of topiramate (TOP) in human plasma by using the liquid–liquid extraction method. The method was developed and validated over the linearity range 15 – 3000 ng/mL with 0.2 mL of human plasma using niclosamide (IS) as an internal standard. The MS – MS ion transitions were monitored at m/z 338.20 – 78.20 and m/z 325.20 - 171.20 for TOP and IS in negative mode respectively. Chromatographic separation was achieved on Gemini C18 (150 mm x 4.6 mm, 5 µm) column with an isocratic mobile phase composed of 2 mM ammonium acetate and acetonitrile in the ratio of 15:85 (v/v), at a flow-rate of 0.5 mL/min. This method demonstrated intra- and inter-day precision within 3.61 – 9.35% and 4.85 – 9.61% and accuracy within 93.09 – 105.24% and 92.46 – 104.40%. The extraction recoveries of TOP were over 89%. TOP was found to be stable throughout three freeze–thaw cycles, bench top and postoperative stability studies. The method is proved to be accurate and speciï¬c, and was successfully applied to the pharmacokinetic study in epileptic patients